@article {2025, title = {Impact of multiple phosphorylations on the tau-R2/tubulin interface}, journal = {Biochemistry}, year = {2025}, abstract = {

The phosphorylation of the microtubule-associated tau protein plays a key role in the regulation of its physiological function. In particular, tau hyperphosphorylation affects its binding to the tubulin surface, destabilizing the tau-microtubule interface and leading to the accumulation of fibrillar aggregates in the brain. In this work, we performed classical molecular dynamics simulations for the tau-R2/tubulin assembly with various phosphorylation states of serines 285, 289, and 293. We analyze the resulting trajectories to obtain a detailed view of the protein interface in the complex and the impact of tau phosphorylations on the stability of this assembly and on the mobility of the tubulin disordered C-terminal tails (CTTs). We show how the tubulin CTTs help maintain the tau-R2 fragment on the tubulin surface despite the destabilizing effect induced by phosphorylations. Conversely, tau phosphorylation affects the CTTs\&$\#$39; flexibility and their potential activity as MAP-recruiting hooks. Furthermore, counterion-mediated bridges between the phosphate groups and tubulin glutamates also contribute to the binding of tau-R2 on the MT. Overall, the complex dynamics of this fuzzy phosphorylated assembly shed new light on the importance of the cytoplasmic environment in neurons in the context of Alzheimer\&$\#$39;s disease.

}, author = {Marien, Jules and Pr{\'e}vost, Chantal and S Sacquin-Mora} } @article {2023|2166, title = {It Takes Tau to Tango: Investigating the Fuzzy Interaction between the R2-Repeat Domain and Tubulin C-Terminal Tails}, journal = {Biochemistry}, volume = {62}, year = {2023}, month = {Aug}, pages = {2492{\textendash}2502}, author = {Marien, J and Prevost, C and S Sacquin-Mora} } @article {2021|2154, title = {Influences of ssDNA-RecA Filament Length on the Fidelity of Homologous Recombination}, journal = {Journal of Molecular Biology}, volume = {433}, year = {2021}, pages = {167143}, abstract = {

Chromosomal double-strand breaks can be accurately repaired by homologous recombination, but genomic rearrangement can result if the repair joins different copies of a repeated sequence. Rearrangement can be advantageous or fatal. During repair, a broken double-stranded DNA (dsDNA) is digested by the RecBCD complex from the 5 {\"A} end, leaving a sequence gap that separates two 3{\"A} single-stranded DNA (ssDNA) tails. RecA binds to the 3 {\"A} tails forming helical nucleoprotein filaments.A three-strand intermediate is formed when a RecA-bound ssDNA with L nucleotides invades a homologous region of dsDNA and forms a heteroduplex product with a length \≤ L bp. The homology dependent stability of the heteroduplex determines how rapidly and accurately homologous recombination repairs double-strand breaks. If the heteroduplex is sufficiently sequence matched, repair progresses to irreversible DNA synthesis. Otherwise, the heteroduplex should rapidly reverse. In this work, we present in vitro measurements of the L dependent stability of heteroduplex products formed by filaments with 90 \≤ L \≤ 420 nt, which is within the range observed in vivo. We find that without ATP hydrolysis, products are irreversible when L \> 50 nt. In contrast, with ATP hydrolysis when L \< 160 nt, products reverse in \< 30 seconds; however, with ATP hydrolysis when L \>= 320 nt, some products reverse in \< 30 seconds, while others last thousands of seconds. We consider why these two different filament length regimes show such distinct behaviors. We propose that the experimental results combined with theoretical insights suggest that filaments with 250 \≤ L \≤ 8500 nt optimize DSB repair.

}, keywords = {biased random walk, D-loop turnover, Double-strand break repair, genomic rearrangement, multiple reversible intermediates}, issn = {0022-2836}, doi = {https://doi.org/10.1016/j.jmb.2021.167143}, url = {https://www.sciencedirect.com/science/article/pii/S0022283621003727}, author = {Claudia Danilowicz and Evan Vietorisz and Veronica Godoy-Carter and Chantal Pr{\'e}vost and Mara Prentiss} } @article {2020|2126, title = {Impact of A2T and D23N Mutations on Tetrameric Aβ42 Barrel within a Dipalmitoylphosphatidylcholine Lipid Bilayer Membrane by Replica Exchange Molecular Dynamics.}, journal = {J Phys Chem B}, volume = {124}, year = {2020}, month = {2020 Feb 20}, pages = {1175-1182}, abstract = {

In Alzheimer\&$\#$39;s disease (AD), many experimental and computational studies support the amyloid pore hypothesis of the Aβ42 peptide. We recently designed a β-barrel tetramer in a membrane-mimicking environment consistent with some low-resolution experimental data. In this earlier study, by using extensive replica exchange molecular dynamics simulations, we found that the wild-type (WT) Aβ42 peptides have a high propensity to form β-barrels, while the WT Aβ40 peptides do not. In this work, we have investigated the effect of mutations D23N and A2T on the Aβ42 barrel tetramer by using the same enhanced conformational sampling technique. It is known that the D23N mutation leads to early onset AD, while the A2T mutation protects from AD. This computational study in a dipalmitoylphosphatidylcholine (DPPC) lipid bilayer membrane shows that the WT sequence and its A2T variant have similar β-barrel populations and the three-dimensional model is slightly destabilized for D23N compared to its WT sequence. These extensive modeling calculations indicate that the lower and higher induced toxicity of these two mutations in AD cannot be correlated to their β-barrel tetramer stabilities in a DPPC lipid bilayer membrane.

}, issn = {1520-5207}, doi = {10.1021/acs.jpcb.9b11881}, author = {Ngo, Son Tung and Phuong Hoang Nguyen and Philippe Derreumaux} } @article {2020|2125, title = {Impact of the Astaxanthin, Betanin, and EGCG Compounds on Small Oligomers of Amyloid Aβ Peptide.}, journal = {J Chem Inf Model}, volume = {60}, year = {2020}, month = {2020 Mar 23}, pages = {1399-1408}, abstract = {

There is experimental evidence that the astaxanthin, betanin, and epigallocatechin-3-gallate (EGCG) compounds slow down the aggregation kinetics and the toxicity of the amyloid-β (Aβ) peptide. How these inhibitors affect the self-assembly at the atomic level remains elusive. To address this issue, we have performed for each ligand atomistic replica exchange molecular dynamic (REMD) simulations in an explicit solvent of the Aβ trimer from the U-shape conformation and MD simulations starting from Aβ dimer and tetramer structures characterized by different intra- and interpeptide conformations. We find that the three ligands have similar binding free energies on small Aβ oligomers but very distinct transient binding sites that will affect the aggregation of larger assemblies and fibril elongation of the Aβ peptide.

}, issn = {1549-960X}, doi = {10.1021/acs.jcim.9b01074}, author = {Minh Hung, Huynh and Nguyen, Minh Tho and Tran, Phuong-Thao and Truong, Vi Khanh and Chapman, James and Quynh Anh, Le Huu and Philippe Derreumaux and Vu, Van V and Ngo, Son Tung} } @article {2020|2144, title = {Implicit Modeling of the Impact of Adsorption on Solid Surfaces for Protein Mechanics and Activity with a Coarse-Grained Representation}, journal = {J Phys Chem B}, volume = {124}, year = {2020}, month = {Oct}, pages = {8516{\textendash}8523}, author = {Bourassin, N. and Marc Baaden and Lojou, E. and S Sacquin-Mora} } @article {2019|2108, title = {Interaction mechanism between the focused ultrasound and lipid membrane at the molecular level}, journal = {The Journal of chemical physics}, volume = {150}, year = {2019}, pages = {215101}, author = {Man, Viet Hoang and Li, Mai Suan and Wang, Junmei and Philippe Derreumaux and Phuong Hoang Nguyen} } @article {2019|2067, title = {An inter-dimer allosteric switch controls NMDA receptor activity}, journal = {The EMBO journal}, volume = {38}, year = {2019}, author = {Esmenjaud, Jean-Baptiste and Stroebel, David and Chan, Kelvin and Grand, Teddy and David, M{\'e}lissa and Wollmuth, Lonnie P and Antoine Taly and Paoletti, Pierre} } @article {2019|2071, title = {Involvement of the GABAA receptor α subunit in the mode of action of etifoxine}, journal = {Pharmacological research}, volume = {145}, year = {2019}, pages = {104250}, author = {Mattei, C{\'e}sar and Antoine Taly and Soualah, Zineb and Saulais, Oph{\'e}lie and Henrion, Daniel and Gu{\'e}rineau, Nathalie C and Verleye, Marc and Legros, Christian} } @article {2018|2034, title = {Influence of electric field on the amyloid-β(29-42) peptides embedded in a membrane bilayer.}, journal = {J Chem Phys}, volume = {148}, year = {2018}, month = {2018 Jan 28}, pages = {045105}, abstract = {

Alzheimer\&$\#$39;s disease is linked to various types of aggregates of amyloid-β (Aβ) peptide and their interactions with protein receptors and neuronal cell membranes. Little is known on the impact of the electric field on membrane-embedded Aβ. Here we use atomistic molecular dynamics simulations to study the effects of a constant electric field on the conformations of Aβdimer inside a membrane, where the electric field has a strength of 20 mV/nm which exists across the membrane of a human neuron. Starting from α-helix peptides, the transmembrane electric field (TMEF) accelerates the conversion from the Gly-out substate to the Gly-side and Gly-in substates. Starting from β-sheet peptides, TMEF induces changes of the kink and tilt angles at Gly33 and Gly37. Overall, in the simulations totaling 10 μs, TMEF establishes new ground states for the dimer, similar to induced-fit in ligand binding. Our findings indicate that TMEF can stabilize rare conformations of amyloid peptides, and this could influence the cleavage of the amyloid precursor protein and the formation of β-sheet oligomers in membrane bilayers.

}, issn = {1089-7690}, doi = {10.1063/1.5018459}, author = {Lu, Yan and Shi, Xiao-Feng and Salsbury, Freddie R and Philippe Derreumaux} } @article {2016|1578, title = {Impact of the A2V Mutation on the Heterozygous and Homozygous A beta 1-40 Dimer Structures from Atomistic Simulations}, journal = {Acs Chem. Neurosci.}, volume = {7}, number = {6}, year = {2016}, month = {jun}, pages = {823{\textendash}832}, abstract = {The A2V mutation was reported to protect from Alzheimer{\textquoteright}s disease in its heterozygous form and cause an early Alzheimer{\textquoteright}s disease type dementia in its homozygous form. Experiments showed that the aggregation rate follows the order A2V > WT (wild-type) > A2V-WT. To understand the impact of this mutation, we carried out replica exchange molecular dynamics simulations of A beta 1-40 WT-A2V and A2V-A2V dimers and compared to the WT dimer. Our atomistic simulations reveal that the mean secondary structure remains constant, but there are substantial differences in the intramolecular and intermolecular conformations upon single and double A2V mutation. Upon single mutation, the intrinsic disorder is reduced, the intermolecular potential energies are reduced, the population of intramolecular three-stranded beta-sheets is increased, and the number of all a dimer topologies is decreased. Taken together, these results offer an explanation for the reduced aggregation rate of the A beta 1-40 A2V-WT peptides and the protective effect of A2V in heterozygotes.}, issn = {1948-7193}, doi = {10.1021/acschemneuro.6b00053}, author = {Phuong Hoang Nguyen and Fabio Sterpone and Campanera, Josep M. and Nasica-Labouze, Jessica and Philippe Derreumaux} } @article {2016|1711, title = {In silico structural characterization of protein targets for drug development against Trypanosoma cruzi}, journal = {J. Mol. Model.}, volume = {22}, number = {10}, year = {2016}, month = {oct}, abstract = {Trypanosoma cruzi is the protozoan pathogen responsible for Chagas disease, which is a major public health problem in tropical and subtropical regions of developing countries and particularly in Brazil. Despite many studies, there is no efficient treatment against Chagas disease, and the search for new therapeutic targets specific to T. cruzi is critical for drug development. Here, we have revisited 41 protein sequences proposed by the analogous enzyme pipeline, and found that it is possible to provide structures for T. cruzi sequences with clear homologs or analogs in H. sapiens and likely associated with trypanothione reductase, cysteine synthase, and ATPase functions, and structures for sequences specific to T. cruzi and absent in H. sapiens associated with 2,4-dienoyl-CoA reductase, and leishmanolysin activities. The implications of our structures refined by atomistic molecular dynamics (monomer or dimer states) in their in vitro environments (aqueous solution or membrane bilayers) are discussed for drug development and suggest that all protein targets, except cysteine synthase, merit further investigation.}, issn = {1610-2940}, doi = {10.1007/s00894-016-3115-9}, author = {Lima, Carlyle Ribeiro and Carels, Nicolas and Ramos Guimaraes, Ana Carolina and Pierre Tuffery and Philippe Derreumaux} } @conference {2016|1415, title = {Interactive visual analytics of molecular data in immersive environments via a semantic definition of the content and the context}, booktitle = {2016 Workshop on Immersive Analytics (IA)}, year = {2016}, month = {March}, abstract = {

Bringing together, in a unique immersive environment, visualization and analysis of scientific and complex data requires a thorough approach in order to fulfill scientists\&$\#$39; specific expectations. Such an approach needs to consider the highly heterogeneous nature of data, the dynamic interactions between experts and data, and the large amount of data involved in scientific studies. Whereas small and static scientific datasets can quickly be deciphered thanks to standard immersive tools such as 3D visualization software packages, bigger and dynamic datasets exceed the analytical capacity of these tools, requiring an efficient platform for their manipulation. Through the example of the structural biology field we discuss the need for an approach based on a high-level definition of the content (scientific data) and the context (immersive environments and interfaces). Our design is illustrated by a platform for dynamic and intelligent representation of data to the user. The data hierarchical classification will provide new ways to interact with the data via intelligent and direct relationships between them. This approach is based on the semantic definition of all the concepts manipulated in the virtual environment, either abstract or concrete, which allows for an adaptive and interactive experience of both visualization and analysis.

}, keywords = {Computer Graphics [I.3.7]: Three-Dimensional Graphics and Realism-Virtual Reality, content high-level definition, content management, content semantic definition, Context, context high-level definition, context semantic definition, data analysis, data dynamic representation, data intelligent representation, data visualisation, Data visualization, molecular data interactive visual analysis, Ontologies, Resource description framework, Semantics, static scientific datasets, structural biology field, Three-dimensional displays, Tools, virtual environment, Virtual Reality}, doi = {10.1109/IMMERSIVE.2016.7932383}, author = {M. Trellet and Nicolas F{\'e}rey and Marc Baaden and P. Bourdot} } @article {2015|1643, title = {Inhibition of protein aggregation and amyloid formation by small molecules}, journal = {Curr. Opin. Struct. Biol.}, volume = {30}, year = {2015}, month = {feb}, pages = {50{\textendash}56}, doi = {10.1016/j.sbi.2014.12.004}, author = {Doig, Andrew J. and Philippe Derreumaux} } @article {2015|1731, title = {Integrating multi-scale data on homologous recombination into a new recognition mechanism based on simulations of the RecA-ssDNA/dsDNA structure}, journal = {Nucleic Acids Res.}, volume = {43}, year = {2015}, month = {dec}, pages = {10251{\textendash}63}, abstract = {

RecA protein is the prototypical recombinase. Members of the recombinase family can accurately repair double strand breaks in DNA. They also provide crucial links between pairs of sister chromatids in eukaryotic meiosis. A very broad outline of how these proteins align homologous sequences and promote DNA strand exchange has long been known, as are the crystal structures of the RecA-DNA pre- and postsynaptic complexes; however, little is known about the homology searching conformations and the details of how DNA in bacterial genomes is rapidly searched until homologous alignment is achieved. By integrating a physical model of recognition to new modeling work based on docking exploration and molecular dynamics simulation, we present a detailed structure/function model of homology recognition that reconciles extremely quick searching with the efficient and stringent formation of stable strand exchange products and which is consistent with a vast body of previously unexplained experimental results.

}, doi = {10.1093/nar/gkv883}, author = {Yang, Darren and Boyer, Benjamin and Chantal Pr{\'e}vost and Danilowicz, Claudia and Prentiss, Mara} } @article {2015|1965, title = {An integrative approach to the study of filamentous oligomeric assemblies, with application to {R}ec{A}}, journal = {Plos One}, volume = {in press}, year = {2015}, pages = {e0116414}, abstract = {

Oligomeric macromolecules in the cell self-organize into a wide variety of geometrical motifs such as helices, rings or linear filaments. The recombinase proteins involved in homologous recombination present many such assembly motifs. Here, we examine in particular the polymorphic characteristics of RecA, the most studied member of the recombinase family, using an integrative approach that relates local modes of monomer/monomer association to the global architecture of their screw-type organization. In our approach, local modes of association are sampled via docking or Monte Carlo simulations. This enables shedding new light on fiber morphologies that may be adopted by the RecA protein. Two distinct RecA helical morphologies, the so-called \"extended\" and \"compressed\" forms, are known to play a role in homologous recombination. We investigate the variability within each form in terms of helical parameters and steric accessibility. We also address possible helical discontinuities in RecA filaments due to multiple monomer-monomer association modes. By relating local interface organization to global filament morphology, the strategies developed here to study RecA self-assembly are particularly well suited to other DNA-binding proteins and to filamentous protein assemblies in general.

}, doi = {10.1371/journal.pone.0116414}, author = {Benjamin Boyer and Johann Ezelin and Pierre Poulain and A Saladin and Martin Zacharias and Charles H. Robert and Chantal Pr{\'e}vost} } @article {2015|1586, title = {Investigating the Structural Variability and Binding Modes of the Glioma Targeting NFL-TBS.40-63 Peptide on Tubulin}, journal = {Biochemistry}, volume = {54}, number = {23}, year = {2015}, month = {jun}, pages = {3660{\textendash}3669}, doi = {10.1021/acs.biochem5b00146}, author = {Laurin, Y. and Savarin, P. and Charles H. Robert and M. Takahashi and Eyer, J. and Chantal Pr{\'e}vost and S Sacquin-Mora} } @article {2014|1411, title = {{I}nnovative interactive flexible docking method for multi-scale reconstruction elucidates dystrophin molecular assembly}, journal = {Faraday Discuss.}, volume = {169}, year = {2014}, note = {[DOI:\href{http://dx.doi.org/10.1039/c3fd00134b}{10.1039/c3fd00134b}] [PubMed:\href{http://www.ncbi.nlm.nih.gov/pubmed/25340652}{25340652}]}, pages = {45{\textendash}62}, author = {Molza, A. E and Nicolas F{\'e}rey and Czjzek, M and Le Rumeur, E and Hubert, J. F and Tek, A and Laurent, B and Marc Baaden and Delalande, O.} } @article {2014|1893, title = {Improved PEP-FOLD Approach for Peptide and Miniprotein Structure Prediction}, journal = {J. Chem. Theory Comput.}, volume = {10}, number = {10}, year = {2014}, month = {oct}, pages = {4745{\textendash}4758}, doi = {10.1021/ct500592m}, author = {Shen, Yimin and Maupetit, Julien and Philippe Derreumaux and Pierre Tuffery} } @article {2014|1772, title = {INRIA Tech Report: Conformational ensembles and sampled landscapes: analysis and comparison.}, year = {2014}, author = {Fr{\'e}d{\'e}ric Cazals and T. Dreyfus and D. Mazauric and A. Roth and Charles H. Robert} } @article {2014|1964, title = {Interface Matters: The Stiffness Route to Stability of a Thermophilic Tetrameric Malate Dehydrogenase}, journal = {Plos One}, volume = {9}, number = {12}, year = {2014}, month = {dec}, pages = {e113895}, url = {http://dx.doi.org/10.1371\%2Fjournal.pone.0113895}, author = {Kalimeri, Maria and Girard, Eric and Madern, Dominique and Sterpone, Fabio} } @article {2013|1892, title = {Importance of the Ion-Pair Interactions in the OPEP Coarse-Grained Force Field: Parametrization and Validation}, journal = {J. Chem. Theory Comput.}, volume = {9}, number = {10}, year = {2013}, month = {oct}, pages = {4574{\textendash}4584}, doi = {10.1021/ct4003493}, author = {Sterpone, Fabio and Phuong Hoang Nguyen and Kalimeri, Maria and Philippe Derreumaux} } @inbook {2013|1533, title = {Inquiring Protein Thermostability: Is Resistance to Temperature Stress a Rigidity/Flexibility Trade-off?}, booktitle = {Proceedings of the European Conference on Complex Systems 2012}, year = {2013}, publisher = {Springer International Publishing}, organization = {Springer International Publishing}, author = {Kalimeri, Maria and Melchionna, Simone and Sterpone, Fabio} } @article {2013|1531, title = {Interactive Molecular Dynamics: Scaling up to Large Systems.}, journal = {Procedia Comput. Sci.}, volume = {18}, year = {2013}, pages = {20{\textendash}29}, doi = {10.1016/j.procs.2013.05.165}, author = {M. Dreher and M. Piuzzi and A. Turki and Matthieu Chavent and Marc Baaden and Nicolas F{\'e}rey and S. Limet and B. Raffin and S. Robert} } @article {2012|1793, title = {{I}ntermediate closed channel state(s) precede(s) activation in the {A}{T}{P}-gated {P}2{X}2 receptor}, journal = {Channels (austin)}, volume = {6}, number = {5}, year = {2012}, pages = {398{\textendash}402}, author = {Jiang, R. and Antoine Taly and Lemoine, D. and Martz, A. and Specht, A. and Grutter, T.} } @article {2012|1756, title = {Involvement of the cysteine-rich head domain in activation and desensitization of the P2X1 receptor}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {109}, number = {28}, year = {2012}, pages = {11396{\textendash}11401}, publisher = {National Acad Sciences}, author = {L{\"o}rinczi, {\'E}va and Bhargava, Yogesh and Marino, Stephen F and Antoine Taly and Kaczmarek-H{\'a}jek, Karina and Barrantes-Freer, Alonso and Dutertre, S{\'e}bastien and Grutter, Thomas and Rettinger, J{\"u}rgen and Nicke, Annette} } @article {2011|1889, title = {Impact of Thermostats on Folding and Aggregation Properties of Peptides Using the Optimized Potential for Efficient Structure Prediction Coarse-Grained Model}, journal = {J. Chem. Theory Comput.}, volume = {7}, number = {5}, year = {2011}, month = {may}, pages = {1502{\textendash}1510}, doi = {10.1021/ct100619p}, author = {Spill, Yannick G. and Pasquali, Samuela and Philippe Derreumaux} } @article {2011|1966, title = {Intrinsic Determinants of A beta(12-24) pH-Dependent Self-Assembly Revealed by Combined Computational and Experimental Studies}, journal = {Plos One}, volume = {6}, number = {9}, year = {2011}, month = {sep}, pages = {e24329}, doi = {10.1371/journal.pone.0024329}, author = {Xu, Weixin and Zhang, Ce and Philippe Derreumaux and Graslund, Astrid and Morozova-Roche, Ludmilla and Mu, Yuguang} } @article {2010|1511, title = {{I}ntrinsic flexibility of {B}-{D}{N}{A}: the experimental {T}{R}{X} scale}, journal = {Nucleic Acids Res.}, volume = {38}, year = {2010}, month = {jan}, pages = {1034{\textendash}1047}, author = {Heddi, B. and Oguey, C. and Lavelle, C. and Foloppe, N. and Hartmann, B.} } @article {2010|1470, title = {Infrared signatures of the peptide dynamical transition: A molecular dynamics simulation study}, journal = {J. Chem. Phys.}, volume = {133}, number = {3}, year = {2010}, month = {jul}, author = {Kobus, Maja and Phuong Hoang Nguyen and Stock, Gerhard} } @conference {2010|1410, title = {Interacting with Molecular Simulations using a Multimodal VR Framework}, booktitle = {EuroVR-EVE}, year = {2010}, pages = {1{\textendash}4}, address = {Orsay, France}, author = {A Tek and B Laurent and Nicolas F{\'e}rey and Marc Baaden} } @book {2010|1571, title = {Itin{\'e}raires Bis. Mon parcours de jeune chercheur : 13 chercheurs du CNRS t{\'e}moignent.}, year = {2010}, publisher = {Connaissance et Savoirs}, organization = {Connaissance et Savoirs}, author = {Collectif (M. Baaden et al.)} } @article {2009|1614, title = {Induced beta-Barrel Formation of the Alzheimer{\textquoteright}s A beta 25-35 Oligomers on Carbon Nanotube Surfaces: Implication for Amyloid Fibril Inhibition}, journal = {Biophys. J.}, volume = {97}, number = {6}, year = {2009}, month = {sep}, pages = {1795{\textendash}1803}, doi = {10.1016/j.bpj.2009.07.014}, author = {Fu, Zhaoming and Luo, Yin and Philippe Derreumaux and Wei, Guanghong} } @article {2009|1387, title = {Induced beta-barrel formation of the Alzheimer{\textquoteright}s Abeta25-35 oligomers on carbon nanotube surfaces: implication for amyloid fibril inhibition.}, journal = {Biophys. J.}, volume = {97}, number = {6}, year = {2009}, month = {sep}, pages = {1795{\textendash}1803}, doi = {10.1016/j.bpj.2009.07.014}, author = {Zhaoming Fu and Yin Luo and Philippe Derreumaux and Guanghong Wei} } @article {2008|1710, title = {Identification of Protein Interaction Partners and Protein-Protein Interaction Sites}, journal = {J. Mol. Biol.}, volume = {382}, number = {5}, year = {2008}, pages = {1276{\textendash}1289}, doi = {10.1016/j.jmb.2008.08.002}, author = {S Sacquin-Mora and Carbone, A. and Richard Lavery} } @article {2008|1439, title = {Importance of accurate DNA structures in solution: the Jun-Fos model.}, journal = {J. Mol. Biol.}, volume = {382}, number = {(4)}, year = {2008}, pages = {956{\textendash}70}, author = {Heddi, B and Foloppe, N and Oguey, C and Hartmann, B} } @article {2008|1662, title = {Insights on protein-DNA recognition by coarse grain modelling}, journal = {J. Comput. Chem.}, volume = {29}, year = {2008}, month = {nov}, pages = {2582{\textendash}92}, abstract = {

Coarse grain modelling of macromolecules is a new approach, potentially well adapted to answer numerous issues, ranging from physics to biology. We propose here an original DNA coarse grain model specifically dedicated to protein-DNA docking, a crucial, but still largely unresolved, question in molecular biology. Using a representative set of protein-DNA complexes, we first show that our model is able to predict the interaction surface between the macromolecular partners taken in their bound form. In a second part, the impact of the DNA sequence and electrostatics, together with the DNA and protein conformations on docking is investigated. Our results strongly suggest that the overall DNA structure mainly contributes in discriminating the interaction site on cognate proteins. Direct electrostatic interactions between phosphate groups and amino acid side chains strengthen the binding. Overall, this work demonstrates that coarse grain modeling can reveal itself a precious auxiliary for a general and complete description and understanding of protein-DNA association mechanisms.

}, doi = {10.1002/jcc.21014}, author = {Poulain, P and A Saladin and Hartmann, B and Chantal Pr{\'e}vost} } @article {2008, title = {Interactions between neuronal fusion proteins explored by molecular dynamics}, journal = {Biophys. J.}, volume = {94}, number = {9}, year = {2008}, month = {may}, pages = {3436{\textendash}3446}, author = {Durrieu, Marie-Pierre and Lavery, Richard and Marc Baaden} } @article {2008|1840, title = {Ion dynamics and water percolation effects in DNA polymorphism}, journal = {J. Am. Chem. Soc.}, volume = {130}, number = {1}, year = {2008}, pages = {121{\textendash}131}, author = {Ivan Brovchenko and Aliaksei Krukau and Alla Oleinikova and Alexey K Mazur} } @article {2006|2008, title = {{I}dentification of two critical residues within the {C}ys-loop sequence that determine fast-gating kinetics in a pentameric ligand-gated ion channel}, journal = {J. Mol. Neurosci.}, volume = {30}, number = {1-2}, year = {2006}, pages = {63{\textendash}64}, publisher = {Springer}, author = {Grutter, T. and de Carvalho, L. P. and Dufresne, V. and Antoine Taly and Jean-Pierre Changeux} } @article {2006|1754, title = {{I}mplications of the quaternary twist allosteric model for the physiology and pathology of nicotinic acetylcholine receptors}, journal = {Proc. Natl. Acad. Sci. U.s.a.}, volume = {103}, number = {45}, year = {2006}, month = {nov}, pages = {16965{\textendash}16970}, author = {Antoine Taly and Corringer, P. J. and Grutter, T. and Prado de Carvalho, L. and Karplus, M. and Jean-Pierre Changeux} } @article {2006|1497, title = {An Immersive Visualization and Interaction Paradigm for Genomic Databases Exploration}, journal = {Mediterranean Journal of Computers and Networks}, volume = {2}, number = {3}, year = {2006}, month = {jul}, pages = {118{\textendash}124}, author = {Nicolas F{\'e}rey and P.-E. Gros and R. Gherbi} } @article {2006|1618, title = {Impact of the mutation A21G (Flemish variant) on Alzheimer{\textquoteright}s beta-amyloid dimers by molecular dynamics simulations}, journal = {Biophys. J.}, volume = {91}, number = {10}, year = {2006}, month = {nov}, pages = {3829{\textendash}3840}, doi = {10.1526/biophysj.106.090993}, author = {Huet, Alexis and Philippe Derreumaux} } @article {2006|1757, title = {Implications of the quaternary twist allosteric model for the physiology and pathology of nicotinic acetylcholine receptors}, journal = {Proceedings of the National Academy of Sciences}, volume = {103}, number = {45}, year = {2006}, pages = {16965{\textendash}16970}, publisher = {National Acad Sciences}, author = {Antoine Taly and Corringer, Pierre-Jean and Grutter, Thomas and De Carvalho, Lia Prado and Karplus, Martin and Jean-Pierre Changeux} } @article {2006|1466, title = {Improved Wang-Landau sampling through the use of smoothed potential-energy surfaces}, journal = {J. Chem. Phys.}, volume = {124}, number = {15}, year = {2006}, month = {apr}, author = {Phuong Hoang Nguyen and Mittag, E and Torda, AE and Stock, G} } @article {2006|1619, title = {Investigating the local flexibility of functional residues in hemoproteins}, journal = {Biophys. J.}, volume = {90}, number = {8}, year = {2006}, pages = {2706{\textendash}2717}, doi = {10.1529/biophysj.105.074997}, author = {S Sacquin-Mora and Richard Lavery} } @article {2005|1405, title = {Immersive graph-based visualization and exploration of biological data relationships}, journal = {Data Sci. J.}, volume = {4}, year = {2005}, month = {mar}, pages = {189{\textendash}194}, author = {Nicolas F{\'e}rey and P.-E. Gros and J. H{\'e}risson and R. Gherbi} } @article {2005|1900, title = {Improved greedy algorithm for protein structure reconstruction}, journal = {J. Comput. Chem.}, volume = {26}, number = {5}, year = {2005}, month = {apr}, pages = {506{\textendash}513}, doi = {10.1002/jcc.20181}, author = {Pierre Tuffery and Guyon, F and Philippe Derreumaux} } @article {2005|1839, title = {Insights into the recognition and association of transmembrane $\alpha$-helices. {T}he free energy of $\alpha$-helix dimerization in glycophorin {A}}, journal = {J. Am. Chem. Soc.}, volume = {127}, number = {23}, year = {2005}, pages = {8478{\textendash}8484}, abstract = {The free energy of alpha-helix dimerization of the transmembrane (TM) region of glycophorin A was estimated from a 125-ns molecular dynamics (MD) simulation in a membrane mimetic. The free energy profile was obtained by allowing the TM helical segments to diffuse reversibly along the reaction pathway. Partition of the potential of mean force into free energy components illuminates the critical steps of alpha-helix recognition and association. At large separations, the TM segments are pushed together by the solvent, allowing initial, but not necessarily native, interhelical interactions to occur. This early recognition stage precedes the formation of native contacts, which is accompanied by a tilt of the helices, characteristic of the dimeric structure. This step is primarily driven by the van der Waals helix-helix interactions. Free energy perturbation calculations of the L75A and I76A point mutations reveal a disruption in helix-helix association due to a loss of favorable dispersion interactions. Additional MD simulations of the native TM dimer and of a single alpha-helix confirm that, prior to association, individual alpha-helices are independently stable, in agreement with the "two-stage" model of integral membrane protein folding.}, doi = {10.1021/ja050581y}, author = {J{\'e}r{\^o}me H{\'e}nin and A. Pohorille and Christophe Chipot} } @article {2004|1825, title = {Identification of the subunit-subunit interface of Xenopus Rad51.1 protein: Similarity to RecA}, journal = {J. Mol. Biol.}, volume = {335}, number = {4}, year = {2004}, month = {jan}, pages = {895{\textendash}904}, abstract = {

Rad51, like its prokaryotic homolog RecA, forms a helical filament for homologous DNA recombination and recombinational DNA repair. Comparison of the three-dimensional structures of human Rad51 and Escherichia coli RecA indicated that the tyrosine residue at position 191 in human Rad51 lies at the centre of a putative subunit-subunit contact interface. We inserted a tryptophan residue as a fluorescent probe at the corresponding position in Xenopus Rad51.1 and found that its fluorescence depended upon the protein concentration, indicating that the residue is truly in the subunit-subunit interface. We also found that 3 M urea, which promoted the dissociation of Rad51 filament without complete unfolding of the protein, exposed the tryptophan residue to solvent. The fluorescence was not modified by binding to DNA and only slightly modified by ATP, indicating that the same site is used for formation of the active ATP-Rad51-DNA filament. The slight changes in fluorescence caused by ATP and ADP suggest that the subunit-subunit contact is altered, leading to the elongation of the filament by these nucleotides, as with the RecA filament. Thus, Rad51 forms filaments by subunit-subunit contact much like RecA does.

}, author = {Selmane, T and Camadro, JM and Conilleau, S and Fleury, F and Tran, V and Chantal Pr{\'e}vost and Takahashi, M} } @conference {2004|1418, title = {Immersive Graph-based Visualization and Exploration of Biological Data}, booktitle = {International CODATA Conference - The Information Society: New Horizons for Science}, year = {2004}, month = {nov}, address = {Berlin - Germany}, author = {Nicolas F{\'e}rey and P.-E. Gros and J. H{\'e}risson and R. Gherbi} } @article {2004|1930, title = {In silico assembly of Alzheimer{\textquoteright}s A beta(16-22) peptide into beta-sheets}, journal = {J. Am. Chem. Soc.}, volume = {126}, number = {37}, year = {2004}, month = {sep}, pages = {11509{\textendash}11516}, doi = {10.1021/ja047286i}, author = {Santini, S and Mousseau, N and Philippe Derreumaux} } @article {2004|1824, title = {Integrating three views of Arf1 activation dynamics}, journal = {J. Mol. Biol.}, volume = {337}, number = {4}, year = {2004}, month = {apr}, pages = {969{\textendash}983}, author = {Robert, Charles H and Cherfils, Jacqueline and Mouawad, Liliane and Perahia, David} } @article {2003|1990, title = {Impact of the tail and mutations G131V and M129V on prion protein flexibility}, journal = {Proteins-structure Function and Genetics}, volume = {51}, number = {2}, year = {2003}, month = {may}, pages = {258{\textendash}265}, doi = {10.1002/prot.10348}, author = {Santini, S and Claude, JB and Audic, S and Philippe Derreumaux} } @article {2002|1875, title = {Insight into protein topology from Monte Carlo simulations}, journal = {J. Chem. Phys.}, volume = {117}, number = {7}, year = {2002}, month = {aug}, pages = {3499{\textendash}3503}, doi = {10.1063/1.1494427}, author = {Philippe Derreumaux} } @article {2001|1461, title = {Induction-independent recruitment of CREB-binding protein to the c-fos serum response element through interactions between the bromodomain and Elk-1}, journal = {J. Biol. Chem.}, volume = {276}, number = {7}, year = {2001}, month = {feb}, pages = {5213{\textendash}5221}, author = {Nissen, LJ and Gelly, JC and Hipskind, RA} } @article {2001|1802, title = {Internal coordinate phase space analysis of macromolecular systems}, journal = {Comput. Theor. Polym. Sci.}, volume = {11}, number = {1}, year = {2001}, pages = {35{\textendash}47}, author = {Alexey K Mazur and Bobby G. Sumpter and Donald W. Noid} } @inbook {2001|1640, title = {Internal coordinate simulation method}, booktitle = {Computational Biochemistry and Biophysics}, year = {2001}, pages = {115{\textendash}131}, publisher = {Marcel Dekker}, organization = {Marcel Dekker}, address = {New York}, author = {Alexey K Mazur}, editor = {Oren M. Becker and Alexander D. MacKerell and Benoit Roux and Masakatsu Watanabe} } @article {2000|1460, title = {Interaction of trivalent lanthanide cations with phosphoryl derivatives, amide, anisole, pyridine and triazine ligands: a quantum mechanics study}, journal = {J. Alloys Compd.}, volume = {303}, year = {2000}, note = {22nd Rare Earth Research Conference, ARGONNE, ILLINOIS, JUL 11-15, 1999}, month = {may}, pages = {104{\textendash}111}, author = {Marc Baaden and Berny, F and Boehme, C and Muzet, N and Schurhammer, R and Wipff, G} } @inbook {2000|1559, title = {Interfacial features of assisted liquid-liquid extraction of uranyl and cesium salts: a molecular dynamics investigation}, booktitle = {ACS Symposium Series 757}, year = {2000}, pages = {71{\textendash}85}, publisher = {Oxford University Press, New York}, organization = {Oxford University Press, New York}, chapter = {Calixarenes for separations}, author = {Marc Baaden and F. Berny and N. Muzet and L. Troxler and G. Wipff}, editor = {G. Lumetta, R.D. Rogers, and A.S. Gopalan} } @article {1999|1856, title = {Internal correlations in minor groove profiles of experimental and computed B-DNA conformations}, journal = {J. Mol. Biol.}, volume = {290}, year = {1999}, pages = {373{\textendash}377}, author = {Alexey K Mazur} } @article {1995|1632, title = {Investigation of the conformational properties of a {$\beta$}-(1-3) branched {$\beta$}-(1-6) heptasacchardie elicitor and its analogues by internal coordinate stochastic dynamics}, journal = {Carbohydr. Res.}, volume = {279}, year = {1995}, pages = {41{\textendash}57}, author = {Michael G. Petukhov and Alexey K Mazur and Lyudmila A. Elyakova} } @article {1993|1996, title = {INFLUENCE OF THE SPECTROSCOPIC POTENTIAL-ENERGY FUNCTION SPASIBA ON MOLECULAR-DYNAMICS OF PROTEINS - COMPARISON WITH THE AMBER POTENTIAL}, journal = {Theochem-journal of Molecular Structure}, volume = {105}, year = {1993}, month = {oct}, pages = {55{\textendash}64}, author = {Philippe Derreumaux and VERGOTEN, G} } @article {1993|1673, title = {Investigation of conformational equilibrium of polypeptides by internal coordinate stochastic dynamics: Met-enkephalin}, journal = {J. Biomol. Struct. Dyn.}, volume = {10}, year = {1993}, pages = {143{\textendash}167}, author = {Vladimir E. Dorofeyev and Alexey K Mazur} } @article {1986|1652, title = {Ion-Molecule reaction in the gas phase part VI Regioselective SN2 reaction from terpenoid diastereoisomeric diols using CI/NH 4+}, journal = {Helv. Chim. Acta}, volume = {69}, year = {1986}, pages = {806{\textendash}815}, abstract = {

A stereospecific and regioselective SN2 mechanism (Walden inversion) is observed during studies involving modified terpenoid epimeric diols in a high-pressure ion source using ammonia as a reagent gas.

}, doi = {10.1002/hlca.19860690408}, author = {Tabet, Jean-Claude and Chantal Pr{\'e}vost and Bouillot, Anne and Bastard, Josette and Manh, Due Do Khae and Tondeur, Yves} }