Charles H. Robert

Structure, dynamics and interactions of biological macromolecules

A protein or a nucleic acid can take on an astronomical number of different conformations. Whether folding to attain a native form in an auto-structuration process at one extreme or unceasingly interconverting between unstructured conformations at the other, the conformational dynamics of these molecules and their interactions lie at the heart of Biology. That, in a nutshell, is one major motivation of my research: understanding Biology, with obvious practical consequences for understanding human health and pathologies and discovering new therapies.

Yet the high dimensionality of conformational spaces (3 times the number of atoms in the molecule) makes understanding — let alone predicting— the biochemical function of a given macromolecule a decidedly non-trivial task. So another motivation is the challenge of gaining insight into these intricately complex biological systems. Here this is attempted by making use of a few recurring elements: physical-chemical reasoning, mathematical analysis, and computer simulation.

Some news (updated sporadically)

Oct 2020. 600 000 hours awarded by the French High Performance Computing resources of GENCI (CINES and TGCC) for our project on nuclear control of gene expression in organelles.

Sept 2020. An article I wrote on reproducibility of scientific code (hydrodynamics calculations and three-dimensional modelling of chromatin fibers) was featured in Nature

Jan 2020. Gave a talk at the Genopole/University of Evry on the codes governing nuclear control of gene expression in chloroplasts and mitochondria

Oct 2019. Welcome to Céline Cattelin who started her Ph.D. thesis on mRNA recognition by alpha-solenoid proteins with Ingrid Lafontaine and myself

Sept 2018. Congratulations to Marion Sisquellas who completed her Master's degree (Univ. Paris) on the dynamics of PPR proteins with respect to mRNA recognition

Selected examples

Collaborations

Frédéric Cazals   Pinak Chakrabarti   Joël Janin   David Perahia   Ingrid Lafontaine

Recent publications

2017
Laurin Y, Eyer J, Robert CH, Prévost C, Sacquin-Mora S..  2017.  Mobility and core-protein binding patterns of disordered C-terminal tails in β-tubulin isotypes.. Biochemistry. 56(12):1746–1756.